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gene chip fluidics station 450  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene chip fluidics station 450
    Gene Chip Fluidics Station 450, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+chip+fluidics+station+450/gene+chip+fluidics+station+450/pmc09518301-87-13-33
    Average 90 stars, based on 1 article reviews
    gene chip fluidics station 450 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Hybridization:

    Article Title: Deciphering the mechanism of Tinospora cordifolia extract on Th17 cells through in-depth transcriptomic profiling and in silico analysis
    Article Snippet: .. After hybridization, microarray chips were washed and stained using the Affymetrix Gene Chip Fluidics Station 450. .. The stained chips were finally scanned using an Affymetrix Gene Chip Scanner 3000 (Affymetrix, CA, United States).

    Article Title: Alginate oligosaccharide protects endothelial cells against oxidative stress injury via integrin-α/FAK/PI3K signaling.
    Article Snippet: Alginate oligosaccharide (AOS) was reported to possess antioxidant and free radical scavenging activities, but the specific effects and mechanisms remain unclear.. We investigated the effects of AOS on H2O2-induced oxidative stress and apoptosis in human umbilical vein endothelial cells (HUVECs) and the associated mechanisms.. HUVECs were treated with 100–800 lM hydrogen peroxide (H2O2) for various periods (12, 24, and 36 h) to establish an in vitro oxidative stress and apoptosis HUVEC model. AOS protects HUVEC cells against oxidative stress-induced apoptosis by decreasing the expression levels of caspase 3 and Bax, and increasing Bcl-2 expression.

    Article Title: Deciphering the mechanism of Tinospora cordifolia extract on Th17 cells through in-depth transcriptomic profiling and in silico analysis.
    Article Snippet: .. After hybridization, microarray chips were washed and stained using the Affymetrix Gene Chip Fluidics Station 450. .. The stained chips were finally scanned using an Affymetrix Gene Chip Scanner 3000 (Affymetrix, CA, United States).

    Microarray:

    Article Title: Deciphering the mechanism of Tinospora cordifolia extract on Th17 cells through in-depth transcriptomic profiling and in silico analysis
    Article Snippet: .. After hybridization, microarray chips were washed and stained using the Affymetrix Gene Chip Fluidics Station 450. .. The stained chips were finally scanned using an Affymetrix Gene Chip Scanner 3000 (Affymetrix, CA, United States).

    Article Title: Combining metaphase cytogenetics with single nucleotide polymorphism arrays can improve the diagnostic yield and identify prognosis more precisely in myelodysplastic syndromes
    Article Snippet: Briefly, 250 ng of patient DNA was digested with Nsp1, amplified with TITANIUM Taq DNA polymerase (Clontech), fragmented with fragmentation reagent, and labelled with biotin end-labelled nucleotides. .. The DNA was hybridised to the microarray for 16 h, washed on the Gene Chip Fluidics Station 450, stained with Gene Chip Stain Reagents, and scanned on the Gene Chip Scanner 3000Dx v.2 (Thermo Scientific, USA). ..

    Article Title: Deciphering the mechanism of Tinospora cordifolia extract on Th17 cells through in-depth transcriptomic profiling and in silico analysis.
    Article Snippet: .. After hybridization, microarray chips were washed and stained using the Affymetrix Gene Chip Fluidics Station 450. .. The stained chips were finally scanned using an Affymetrix Gene Chip Scanner 3000 (Affymetrix, CA, United States).

    Staining:

    Article Title: Deciphering the mechanism of Tinospora cordifolia extract on Th17 cells through in-depth transcriptomic profiling and in silico analysis
    Article Snippet: .. After hybridization, microarray chips were washed and stained using the Affymetrix Gene Chip Fluidics Station 450. .. The stained chips were finally scanned using an Affymetrix Gene Chip Scanner 3000 (Affymetrix, CA, United States).

    Article Title: Combining metaphase cytogenetics with single nucleotide polymorphism arrays can improve the diagnostic yield and identify prognosis more precisely in myelodysplastic syndromes
    Article Snippet: Briefly, 250 ng of patient DNA was digested with Nsp1, amplified with TITANIUM Taq DNA polymerase (Clontech), fragmented with fragmentation reagent, and labelled with biotin end-labelled nucleotides. .. The DNA was hybridised to the microarray for 16 h, washed on the Gene Chip Fluidics Station 450, stained with Gene Chip Stain Reagents, and scanned on the Gene Chip Scanner 3000Dx v.2 (Thermo Scientific, USA). ..

    Article Title: An extracellular matrix stiffness-induced breast cancer cell transcriptome resembles the transition from ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC).
    Article Snippet: .. The arrays were washed and stained in a Gene Chip Fluidics Station 450 and finally scanned in an Affymetrix Gene Chip Scanner 3000 7G. ..

    Article Title: Decreased mitochondrial‐related gene expression in adipose tissue after acute sprint exercise in humans: A pilot study
    Article Snippet: .. Arrays were hybridized for 16 h at 45°C in a Gene Chip Oven 640 (Affymetrix, Santa Clara, CA, USA) followed by washing and staining using the Gene Chip Fluidics Station 450. .. Scanning was performed using the Affymetrix Gene Chip Scanner 3000 7G.

    Article Title: Electrical stimulation mitigates muscle degradation shift in gene expressions during 12-h mechanical ventilation
    Article Snippet: .. The arrays were washed and stained with a Gene Chip Fluidics Station 450 and scanned on an Affymetrix Gene Chip Scanner 3000 7G using Command Console Software. .. The signal values of the Gene Chip array were normalized based on the total intensity of the array using the SST-RMA algorithm implemented in Transcriptome Analysis Console (TAC) v4.0 software (Thermo Fisher Scientific, MA, USA).

    Article Title: Deciphering the mechanism of Tinospora cordifolia extract on Th17 cells through in-depth transcriptomic profiling and in silico analysis.
    Article Snippet: .. After hybridization, microarray chips were washed and stained using the Affymetrix Gene Chip Fluidics Station 450. .. The stained chips were finally scanned using an Affymetrix Gene Chip Scanner 3000 (Affymetrix, CA, United States).

    Software:

    Article Title: Electrical stimulation mitigates muscle degradation shift in gene expressions during 12-h mechanical ventilation
    Article Snippet: .. The arrays were washed and stained with a Gene Chip Fluidics Station 450 and scanned on an Affymetrix Gene Chip Scanner 3000 7G using Command Console Software. .. The signal values of the Gene Chip array were normalized based on the total intensity of the array using the SST-RMA algorithm implemented in Transcriptome Analysis Console (TAC) v4.0 software (Thermo Fisher Scientific, MA, USA).



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